LOCUS Exported 8101 bp ds-DNA circular SYN 27-MAR-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS portmage4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8101) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, March 27, 2017 from SnapGene Viewer 3.1.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8101 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature complement(149..1996) /label=mutL E32K /note="mutL E32K" misc_feature complement(2014..2694) /label=exo /note="exo" misc_feature complement(2691..3476) /label=bet /note="bet" misc_feature complement(3482..3778) /label=gam /note="gam" promoter complement(3848..3892) /label=pL promoter /note="pL promoter" misc_feature complement(4516..5175) /label=cat /note="cat" misc_feature complement(5536..6249) /label=cI repressor /note="cI repressor" misc_feature 6383..8101 /label=pBBRI origin /note="pBBRI origin" ORIGIN 1 cagctgtctc ttatacacat ctccgctgtg ctttcagtgg atttcggata acagaaaggc 61 cgggaaatac ccagcctcgc tttgtaacgg agtagacgaa agtgattgcg cctacccgga 121 tattatcgtg aggatgctca tcgccatttc actcatcttt cagggctttt atcgccggat 181 gtaaatcaac agattgtaac agaccacccg gcggcgtttt cacaagttgc ggacataacc 241 gttccacgtc cgccagcagg gttatggcct gtgccattga ccactgcgca tgttcgctca 301 tcagatttcg tgcaatccac tgcgcaatat tgccaggttc gaatacggac tgcttcgcca 361 ggtagcctat cagttcagga atcaagattt gtaaattttg ttggcgtaag ggtaaaggca 421 ctgccctgat ggtcacatgc tgtgcatctg actggaaatc aatacccaat tccgccaggg 481 cagactgcgc tttttctaat gccgattttt cttcggcaga aacttttagc cgcaacggaa 541 tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc tgacgcagcc 601 aacgttctgc cactggcaag gataaaagtg aaatgttgcc gtcgcgctcc agcaacgcac 661 agtcggaatg gacgatagtc agtacccgac caaaactctg actgttcgcc gcaagtgcag 721 gttcctgcgg ttccggcgct tttaattttt gcatcggcgc gggcgtttgc aaaagctggc 781 gatacacttc accttgctgt ttctggtagc ctggctgcgc attcggccag ggggcagccg 841 gacgactgcc tgatgctggc gcaggagtgt agcgcggagc taccggctca cgagctgccg 901 gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc cggaatggaa cgcggtgcag 961 gttggggttc atcgtccagc ggtagcggcg tttccagttg ctgttgtagc acgctcagca 1021 cgccctgata gataaaatca tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg 1081 cggggtgcac gttgacgtcc acctgatgtg ggtcgatctc cagatacaac acaaatgccg 1141 gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg gatcgcgtga ttgatcaggc 1201 gatcgcgcat catgcgaccg ttcacgtagc aatactgaat ttctgccagt gcgggcgtgg 1261 tgtgatttgg atcggccacc cagccgcgta gcgtgagatc gccgtgttgc cattcaatcg 1321 ccagcgcttg ttcaagaaaa gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc 1381 cgccttccgg cactgcgcgg tactgacgca caattttacc gttatgcgac aggttgatcg 1441 tgacgtcgaa acgcgccagc gcaatgcggc ggatgatctc atcaatgtgg ttaaattcgg 1501 ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct 1561 ccagcgtcgt ccccacagga tgcgccgccg gttttaccgt cacgttcata tcgcgccctt 1621 cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg 1681 aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc caggctgata atggcttcga 1741 gatcgtccag agaggcgatt ttactggtgg catgacgagc cagcgccagc gccagctcat 1801 cttttttgat accgcagccg ttatcacgaa tgcggataag tttcgcccca ccgcgttcga 1861 tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt tttcactagt tctttgacta 1921 ccgacgcagg tcgctcgacc acctcacctg cggcaatctg gttcgccagt tgtggcggta 1981 agacctgaat tggcattttg taatcctcct ctctcatcgc cattgctccc caaatacaaa 2041 accaatttca gccagtgcct cgtccatttt ttcgatgaac tccggcacga tctcgtcaaa 2101 actcgccatg tacttttcat cccgctcaat cacgacataa tgcaggcctt cacgcttcat 2161 acgcgggtca tagttggcaa agtaccaggc attttttcgc gtcacccaca tgctgtactg 2221 cacctgggcc atgtaagctg actttatggc ctcgaaacca ccgagccgga acttcatgaa 2281 atcccgggag gtaaacgggc atttcagttc aaggccgttg ccgtcactgc ataaaccatc 2341 gggagagcag gcggtacgca tactttcgtc gcgatagatg atcggggatt cagtaacatt 2401 cacgccggaa gtaaattcaa acagggttct ggcgtcgttc tcgtactgtt ttccccaggc 2461 cagtgcttta gcgttaactt ccggagccac accggtgcaa acctcagcaa gcagggtgtg 2521 gaagtaggac attttcatgt caggccactt ctttccggag cggggttttg ctatcacgtt 2581 gtgaacttct gaagcggtga tgacgccgag ccgtaatttg tgccacgcat cttccccctg 2641 ttcgacagct ctcacatcga tcccggtacg ctgcaggata atgtccggtg tcatgctgcc 2701 accttctgct ctgcggcttt ctgtttcagg aatccaagag cttttactgc ttcggcctgt 2761 gtcagttctg acgatgcacg aatgtcgcgg cgaaatatct gggaacagag cggcaataag 2821 tcgtcatccc atgttttatc cagggcgatc agcagagtgt taatctcctg catggtttca 2881 tcgttaaccg gagtgatgtc gcgttccggc tgacgttctg cagtgtatgc agtattttcg 2941 acaatgcgct cggcttcatc cttgtcatag ataccagcaa atccgaaggc cagacgggca 3001 cactgaatca tggctttatg acgtaacatc cgtttgggat gcgactgcca cggccccgtg 3061 atttctctgc cttcgcgagt tttgaatggt tcgcggcggc attcatccat ccattcggta 3121 acgcagatcg gatgattacg gtccttgcgg taaatccggc atgtacagga ttcattgtcc 3181 tgctcaaagt ccatgccatc aaactgctgg ttttcattga tgatgcggga ccagccatca 3241 acgcccacca ccggaacgat gccattctgc ttatcaggaa aggcgtaaat ttctttcgtc 3301 cacggattaa ggccgtactg gttggcaacg atcagtaatg cgatgaactg cgcatcgctg 3361 gcatcacctt taaatgccgt ctggcgaaga gtggtgatca gttcctgtgg gtcgacagaa 3421 tccatgccga cacgttcagc cagcttccca gccagcgttg cgagtgcagt actcattcgt 3481 tttatacctc tgaatcaata tcaacctggt ggtgagcaat ggtttcaacc atgtaccgga 3541 tgtgttctgc catgcgctcc tgaaactcaa catcgtcatc aaacgcacgg gtaatggatt 3601 ttttgctggc cccgtggcgt tgcaaatgat cgatgcatag cgattcaaac aggtgctggg 3661 gcaggccttt ttccatgtcg tctgccagtt ctgcctcttt ctcttcacgg gcgagctgct 3721 ggtagtgacg cgcccagctc tgagcctcaa gacgatcctg aatgtaataa gcgttcatgg 3781 ctgaactcct gaaatagctg tgaaaatatc gcccgcgaaa tgccgggctg attagtaatc 3841 cggaatcgca cttacggcca atgcttcgtt tcgtatcaca caccccaaag ccttctgctt 3901 tgaatgctgc ccttcttcag ggcttaattt ttaagagcgt caccttcatg gtggtcagtg 3961 cgtcctgctg atgtgctcag tatcaccgcc agtggtattt atgtcaacac cgccagagat 4021 aatttatcac cgcagatggt tatctgtatg ttttttatat gaatttattt tttgcagggg 4081 ggcattgttt ggtaggtgag agatctgaat tgctatgttt agtgagttgt atctatttat 4141 ttttcaataa atacaattgg ttatgtgttt tgggggcgat cgtgaggcaa agaaaacccg 4201 gcgctgaggc cgggttaaga gttggtagct cttgatccgg caaacaaacc accgctggta 4261 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 4321 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 4381 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 4441 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttaa ggcaccaata 4501 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt 4561 aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg 4621 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa 4681 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga 4741 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca 4801 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca 4861 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc 4921 ccatatcacc agctcaccgt ctttcattgc catacggaat ttcggatgag cattcatcag 4981 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt 5041 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg 5101 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt 5161 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac 5221 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac 5281 gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt 5341 tatttattct gcgctcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 5401 catgagcgga tacatatttg aatgaattca accatttact atgttatgtt ctgaggggag 5461 tgaaaattcc cctaattcga tgaagattct tgctcaattg ttatcagcta tgcgccgacc 5521 agaacacctt gccgatcagc caaacgtctc ttcaggccac tgactagcga taactttccc 5581 cacaacggaa caactctcat tgcatgggat cattgggtac tgtgggttta gtggttgtaa 5641 aaacacctga ccgctatccc tgatcagttt cttgaaggta aactcatcac ccccaagtct 5701 ggctatgcag aaatcacctg gctcaacagc ctgctcaggg tcaacgagaa ttaacattcc 5761 gtcaggaaag cttggcttgg agcctgttgg tgcggtcatg gaattacctt caacctcaag 5821 ccagaatgca gaatcactgg cttttttggt tgtgcttacc catctctccg catcaccttt 5881 ggtaaaggtt ctaagctcag gtgagaacat ccctgcctga acatgagaaa aaacagggta 5941 ctcatactca cttctaagtg acggctgcat actaaccgct tcatacatct cgtagatttc 6001 tctggcgatt gaagggctaa attcttcaac gctaactttg agaatttttg taagcaatgc 6061 ggcgttataa gcatttaatg cattgatgcc attaaataaa gcaccaacgc ctgactgccc 6121 catccccatc ttgtctgcga cagattcctg ggataagcca agttcatttt tctttttttc 6181 ataaattgct ttaaggcgac gtgcgtcctc aagctgctct tgtgttaatg gtttcttttt 6241 tgtgctcata cgttaaatct atcaccgcaa gggataaata tctaacaccg tgcgtgttga 6301 ctattttacc tctggcggtg ataatggttg catgtactaa ggaggttgta tggaacaacg 6361 agatgtgtat aagagacagc tggcctgccc ctcccttttg gtgtccaacc ggctcgacgg 6421 gggcagcgca aggcggtgcc tccggcgggc cactcaatgc ttgagtatac tcactagact 6481 ttgcttcgca aagtcgtgac cgcctacggc ggctgcggcg ccctacgggc ttgctctccg 6541 ggcttcgccc tgcgcggtcg ctgcgctccc ttgccagccc gtggatatgt ggacgatggc 6601 cgcgagcggc caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc 6661 tgatggacag gctgcgcctg cccacgagct tgaccacagg gattgcccac cggctaccca 6721 gccttcgacc acatacccac cggctccaac tgcgcggcct gcggccttgc cccatcaatt 6781 tttttaattt tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt 6841 tggacaccaa gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt 6901 gacgcgcctg gaacgaccca agcctatgcg agtgggggca gtcgaagggc gaagcccgcc 6961 cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt ccaagggggc agcgccacct 7021 tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc ggaggggcca ctttttgccg 7081 gagggggagc cgcgccgaag gcgtggggga accccgcagg ggtgcccttc tttgggcacc 7141 aaagaactag atatagggcg aaatgcgaaa gacttaaaaa tcaacaactt aaaaaagggg 7201 ggtacgcaac agctcattgc ggcacccccc gcaatagctc attgcgtagg ttaaagaaaa 7261 tctgtaattg actgccactt ttacgcaacg cataattgtt gtcgcgctgc cgaaaagttg 7321 cagctgattg cgcatggtgc cgcaaccgtg cggcacccct accgcatgga gataagcatg 7381 gccacgcagt ccagagaaat cggcattcaa gccaagaaca agcccggtca ctgggtgcaa 7441 acggaacgca aagcgcatga ggcgtgggcc gggcttattg cgaggaaacc cacggcggca 7501 atgctgctgc atcacctcgt ggcgcagatg ggccaccaga acgccgtggt ggtcagccag 7561 aagacacttt ccaagctcat cggacgttct ttgcggacgg tccaatacgc agtcaaggac 7621 ttggtggccg agcgctggat ctccgtcgtg aagctcaacg gccccggcac cgtgtcggcc 7681 tacgtggtca atgaccgcgt ggcgtggggc cagccccgcg accagttgcg cctgtcggtg 7741 ttcagtgccg ccgtggtggt tgatcacgac gaccaggacg aatcgctgtt ggggcatggc 7801 gacctgcgcc gcatcccgac cctgtatccg ggcgagcagc aactaccgac cggccccggc 7861 gaggagccgc ccagccagcc cggcattccg ggcatggaac cagacctgcc agccttgacc 7921 gaaacggagg aatgggaacg gcgcgggcag cagcgcctgc cgatgcccga tgagccgtgt 7981 tttctggacg atggcgagcc gttggagccg ccgacacggg tcacgctgcc gcgccggtag 8041 cacttgggtt gcgcagcaac ccgtaagtgc gctgttccag actatcggct gtaccgcctc 8101 g //